Ribonucleic acid synthesis by Escherichia coli C3000/L after infection by the ribonucleic acid coliphage ZIK/1, and properties of coliphage-ZIK/1 ribonucleic acid.
نویسنده
چکیده
1. A method is described for the preparation and purification of the RNA from the RNA coliphage ZIK/1. 2. Some of the physical characteristics and infective properties of coliphage-ZIK/1 RNA were examined. 3. A method is also described for examining the type and quantity of RNA synthesized after bacteriophage infection. 4. Ribosome synthesis was decreased 15min. after bacteriophage adsorption, bacteriophage RNA was synthesized from 15min. to 120min. after adsorption and intracellular bacteriophages appeared 40min. after adsorption. Cell lysis commenced 60min. after adsorption, and was half complete 20min. later and 90-95% complete 120min. after adsorption. 5. Cell division continued until 40min. after bacteriophage adsorption. 6. Bacterial ribosomes were conserved during the infective process. 7. Intracellular bacteriophage RNA has sedimentation coefficient 28s but after cell lysis it has sedimentation coefficient 10-5s.
منابع مشابه
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Cohen (1948a) has reported that during the multiplication of the bacteriophages T2r+ and T4r+ in Escherichia coli, strain B, the ribonucleic acid content of the host remains constant. Similar observations were made by Kozloff et al. (1951) who investigated the origin of the nitrogen of T6r+ multiplying in the same host. Cohen (1948b) also examined the possibility that the ribonucleic acid was u...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 97 1 شماره
صفحات -
تاریخ انتشار 1965